HPLC - Pharma Egg

Jan142022 by KBS.1 Comment

Operational Qualification of HPLC (High Performance Liquid Chromatography)

In this post we learn Operational Qualification of HPLC (High Performance Liquid Chromatography) in Pharmaceuticals Quality Control Laboratory. 1.0 OBJECTIVE To assure that the equipment used for analysis, is operated, and qualified according to the specifications mentioned in the equipment catalog and complies with company and regulatory standards and guidance Operational Qualification of HPLC (High Performance Liquid Chromatography). 2.0 SCOPE: This protocol applies only to “High-Performance Liquid Chromatograph 1260 series” for its Operational qualification. Qualification of support utilities is not within the scope of this qualification protocol. 3.0 REFERENCES Validation manual/ Validation Master Plan SOP for QA/ QC Equipment Validation Operator’s Manual 4.0 EQUI...

Jan142022 by KBS.1 Comment

Installation Qualification of HPLC (High Performance Liquid Chromatography)

General Topics

In this blogpost we study about Installation Qualification of HPLC. All testing methods presented in this qualification protocol will be validated and confirmed in accordance with the approved procedure. The following signatures imply acceptance of the result. 1.0 Objective: Make sure the goods used for analyzes are determined in accordance with the claims, according to the requirements in accordance and regulatory measures. 2.0 SCOOP: This protocol applies to "DLC-20 HPLC" for the ability to the competence of the installation and input. 3.0 Reference Guide: 3.0 REFERENCES: Validation Manual/ Validation Master Plan. SOP for Equipment Qualification. Instrument’s User’s Manual. 4.0 users The following requires the following for the device: Bayless Karris: • Wavely: Up t...

Jan42022 by KBS.No Comments

HPLC Waters Pda With Quartnary Pump – SOPs

SOPs

This SOP HPLC WATERS PDA WITH QUARTNARY PUMP most important who gives a detailed outline for the operation of HPLC 1 Waters PDA With Quartnary Pump and will cover the operating procedure, calibration and cleaning of equipment. PURPOSE: To describes the operating procedure for operation of HPLC 1 Waters PDA With Quartnary Pump. SCOPE: This SOP gives detailed outline for the operation of HPLC 1 Waters PDA With Quartnary Pump and will cover operating procedure, calibration and cleaning of equipment. RESPONSIBILITY: QC Analyst is responsible for the operation of equipment and preparing standard operating procedures. It is the responsibility of QC Manager to assist and ensure Operating Procedure as per SOP and to make certain that this SOP is ...

Jan32022 by KBS.No Comments

Washing Of HPLC Column SOP

SOPs

Here is the WASHING OF HPLC COLUMN, it is very important in the laboratory, if not done can correctly affect the health of your column. Cleaning your HPLC column may be simple, but it is very important for efficiency, lifetime, and productivity instruments. The ineffective HPLC column can affect your analysis, resulting in inaccurate results, which means more time spent on the same project. The following SOP focuses only on how to clean your HPLC column through reverse flushing. It may be time to water your HPLC column when you see high back pressure, low efficiency, split peak, or the most common problem, the particulate buildup in the head of the column. Now we move to Washing Of HPLC Column SOP Objective: This method is applicable for washing of HPLC column. Sco...

Jan32022 by KBS.1 Comment

Operational SOPs for HPLC (High Performance Liquid Chromatography)

SOPs

In this post, we will study Operational SOPs for HPLC (High Performance Liquid Chromatography). All the related outline, scope, responsiblity and procedure you will study here. 1. PURPOSE To outline work instruction for “HPLC” in Quality Control Department. 2. SCOPE To separation of different material in solution in Quality Control Department. 3. RESPONSIBILITY All analysts are responsible to follow the instruction. 4. PROCEDURE 4.1 Instrument Information Name HPLC Identification No. Model (Pump) L-2100/2130 Model (UV-VIS Detector) L-2420 Range wave length 190-900 nm Operating Temp. Range 15 ºC to 25 ºC 4.2 Method of Operation Connect the instrument to power supply. Switch on...

Jan12022 by KBS.2 Comments

HPLC (High Performance Liquid Chromatography) use in Pharmaceuticals

Quality Control

HPLC use in Pharmaceuticals HPLC is a liquid chromatography form commonly used in the pharmaceutical industry, because it can provide the exact results needed. HPLC (High Performance Liquid Chromatography) use in Pharmaceuticals is increasing day by day. The results can be used to analyze drug products and the materials are quantitative and qualitatively during the manufacturing process. Liquid chromatography is an analytical technique used widely in the pharmaceutical industry. How does it work and what method can be combined to increase its use? High performance liquid chromatography (HPLC) is a very useful analytical technique with various applications. HPLC involves the adsorbent material column (such as silica beads) where the sample is forwarded under pressure. Each sample mixtur...